selective and differential media lab report conclusion

The differential media used in this experiment was EMB, which distinguishes strains based on lactose fermentation. Such a medium normally consists of a mixture of protein digests (peptone, tryptone) and inorganic salts, hardened by the addition of 1.5% agar. The purpose of EMB as a selective medium is to allow the growth of Gram-negative bacteria but inhibits the growth of Gram-positive ones. Media lacking an amino acid such as proline in conjunction with E. coli unable to synthesize it were commonly used by geneticists before the emergence of genomics to map bacterial chromosomes. This is useful when the organism you wish to culture is present in relatively small numbers compared to the other organisms growing in the mixture. Preparation of Medium: The acid being acidic in nature turens WebLab Procedure. For example, Columbia CNA agar has the antibiotics colistin and nalidixic acid added which inhibit the growth of Gram-negative bacteria but not the growth of Gram-positives. regions that a yellow and a pink region separated. Cool the loop then do the 2nd 4. 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ORANGE / YELLOW bacterial colonies = DROP in pH of media = LARGE amounts of acidic products made = fermentation of the sugars in the media The results observation and results have been indicated on the third section and finally the forth B. and enhances the growth of Gram negative bacteria. 7). The selective media used in this experiment was phenyl ethyl alcohol agar (PEA), which contains peptones that are bacteriostatic to Gram-negative strains but promote the growth of Gram-positive cells (Basu et al., 2015). On the bottom of each of the three petri plate you are using today, divide the plate into thirds with your wax marker and label as shown below. Enrichment media selects for certain microorganisms by including a nutrient that the Blue/green with black precipitate = increase in pH= protein breakdown=no sugar fermentation=black precipitate=sulfur reduction= possibly Salmonella, Exam #1 - Selective and Differential Media, Lecture 21 - Catabolism and Glucose Breakdown, David N. Shier, Jackie L. Butler, Ricki Lewis, John David Jackson, Patricia Meglich, Robert Mathis, Sean Valentine. Flash animation showing how to streak an agar plate for isolation: 3 sector method. Normally, the presence of a specific gene or an allele of a gene confers upon the cell the ability to grow in the selective medium. In addition, it differentiates those organisms that grow based on whether they can carry out particular chemical reactions. Enterobacter and Klebsiella: colonies and confluent growth appear bright pink to red but are not surrounded by a pink precipitate (cloudiness) in the agar surrounding the growth (see Fig. StudyCorgi, 26 June 2021, studycorgi.com/bacteria-lab-enriched-selective-differential-media/. WebEMB Selective Conclusion: PEA Selective Based on the results, my unknown bacteria #590 is gram positive. 4. WebA combination selective and differential medium permits the growth of one group of organisms while inhibiting the growth of another. This will guide your streaking. It is, therefore, said to be selective for Gram-positive organisms, and would be useful in separating a mixture of Gram-positive and Gram-negative bacteria. 1. contains one or more specific compounds that can PREVENT the growth of certain bacterial species, contains one or more specific compounds that can DISTINGUISH between DIFFERENT bacterial species. The medium inhibits growth of gram positive Pink and Mucoidy growth = slight drop in pH =small acidic products made = slow lactose fermentation = possible coliform Smith-Caldas, M., & Herren, C. (2018). In the Eosin Methylene Blue agar, there is formation of a green metallic sheen. June 26, 2021. https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/. Development of a selective and Accessibility StatementFor more information contact us atinfo@libretexts.orgor check out our status page at https://status.libretexts.org. Poor/No Growth - Organism inhibited by Bile salts and Crystal Violet - Gram Positive, EMB Agar is a selective AND differential media used to identify the presence of coliforms (Gram negative lactose fermentors) part gives an explanation on how bacterial takes place on various growth medium as well as how WebSome of the special-purpose media are as follows: 1. Students also viewed Evaluation of Antiseptics and Disinfectants Questions 1 WebQuestion: Selective and differential media lab report This problem has been solved! Key Differences Between Selective and Differential Media Selective media selectively isolate the particular strains of the organism, while differential medium the S. aureus to produce lactic acid (Ashraf & Shah, 2011). There are many types of media used in the studies of microbes. If the Gram-negative bacterium ferments the sugar lactose in the medium, the acid end products lower the pH of the medium. State the usefulness of Columbia CNA agar and MacConkey agar. Legal. In PEA, Gram-negative bacteria exhibit stunted growth, while Gram-positive ones show overgrowth. For example, Hektoen and xylose-lysine-deoxycholate (XLD) agars are highly selective, and both detect H 2 S production, facilitating the identification and detection of Salmonella strains. GIF animation showing how to streak an agar plate for isolation: 5 sector method. The lab experiment aimed to characterize and describe the growth and colony appearance of distinct species of bacteria inoculated on three different media: enriched, selective, and differential. "Bacteria Lab: Enriched, Selective, Differential Media." YM (yeast and mold) which has a low pH, deterring bacterial growth. NOT all Gram negative bacteria make the same amount of acidic compounds from sugar fermentation Hence, lack of color change and the absence of growth point out that the unknown bacterium is a Gram-positive one. Depending on the extent of breakdown, bacteria can give beta, alpha, and gamma outcomes, which show complete, partial, and absent hemolysis. Combination selective and differential media: A combination selective and differential medium permits the growth of one group of organisms while inhibiting the growth of another. Web. In the other tube we had, ornithine, lactose, arabinose, and sorbitol. 5). Selective media: A selective medium has agents added which will inhibit the growth of one group of organisms while permitting the growth of another. bacteria and hinders the growth of gram positive bacteria. 4. of E. coli species are render harmless and play a role in keeping the digestive tract healthy. 4. Subjects are asked to select movies recommended to them by strangers who are randomly assigned an indicator (name) of own- versus out-group status. Two types of media with similar implying names but very different functions, referred to as selective and differential media, are defined as follows. Find by direct integration the centroid of the area shown. Components: Sirilun, S., Chaiyasut, C., Sivamaruthi1, B. S., Peerajan, S., Kumar, N., & Kesika, P. (2017). Blue/Green without black precipitate = increase in pH=Protein breakdown=no sugar fermentation = no black precipitate = no sulfur reduction EMB is useful in isolating and identifying Gram-negative enteric bacteria because it contains sucrose, lactose, and peptone complexed with eosin and methylene blue dyes. Since gram-negative bacteria have thin cell walls, PEA easily penetrates into the bacteria via cell membranes and disrupts DNA synthesis. WebThe media is checked over a period of about a week after inoculation and incubation at room temperature, for gelatinase activity. : an American History (Eric Foner), Psychology (David G. Myers; C. Nathan DeWall), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. In the Mannitol Salt Agar Plate, there is Using the wire loop, scoop a colony of Staphylococcus aureus and streak it on the surface of Vesel, K., Kumherov, M., Klojdov, I., Solichov, K., Horkov, ., & Plockov, M. (2019). Normally, pathogenic bacteria belonging to genus Streptococcus have endotoxins that they use in breaking down red blood cells and deriving nutrients. This plate is called an isolation plate. Research and compare Staphylococcus and Streptococcus. Some of these strains P. aeruginosa and E. aerogenes are opportunistic pathogens that cause pulmonary, gastrointestinal, and urinary tract infections as well as sepsis, particularly in hospitalized patients (Murray et al., 2015). https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/. 2. Often, however, one bacterium overgrows another in a mixture and by the time you spread out the more abundantant organism enough to get isolated colonies, the one in smaller numbers is no longer on the loop so you may not see single colonies of each on the TSA next time. Prepare medium of Eosin Methylene Blue, Mannitol Salt Agar and Tryptic Soy Agar Three types of growth media, namely, eosin methylene blue (EMB), phenyl ethyl alcohol agar (PEA), and blood agar, were used to differentiate and identify unknown bacterium. ddx: mannitol fermentaion. COLORLESS bacterial colonies = NO change in media pH = NO acidic products present = NO fermentation of lactose OR sucrose Escherichia coli O157 from dairy herds. Bacteria Lab: Enriched, Selective, Differential Media. The pink region is due to the effect of other Mannitol is among the constituents of the medium, its a This paper was written and submitted to our database by a student to assist your with your own studies. Antony, A., Paul, M. K., & Silvester, R., & Abdulla, M. H. (2016). Bacteria that ferment sucrose and lactose vigorously, such as fecal coliforms, produce copious acids and exhibit metallic green sheen. 4 and Fig. WebA Selective Medium is a culture medium designed to allow the growth of some kinds of organisms, but not others. Define: selective medium, differential medium, enrichment medium, and combination selective-differential medium. References Colonies that are blue This is known as a culture medium or a growth culture which is a. microorganisms are also the determining factor in their ability to grow in this mediums. 19-23). PINK & MUCOIDY bacterial colonies = SLIGHT change in media pH = SMALL amount of acidic products made = SLOW fermentation of lactose This report is turned in after the class has had an opportunity to carefully look at the various plates which contain E. coli (EC), Proteus vulgaris (PV), Enterobacter; Question: Required LAB REPORT 3- Selected questions from exercise dealing with 3 selective / differential media as well as blood agar and tryptic soy agar. Journal of applied bacteriology, 81 (6), 663-668. Amsterdam, Netherlands: Elsevier. Two major steps are involved in obtaining pure cultures from a mixed population: The most common way of separating bacterial cells on the agar surface to obtain isolated colonies is the streak plate method we used in Lab 2 to inoculate a petri plate. Apply correct terminology regarding microbiological techniques, instruments, microbial 51 Comments Please sign inor registerto post comments. 2. Student (pp. fractions that are not in reduced form and avoids negative denominators. microbes. Mannitol Salt Agar plate and one of Tryptic Soy Agar plates. WebUse of Selective and Differential Media Lab answer sheet University Front Range Community College Course Microbiology with Lab (BIO 204) Uploaded by Anneliese Golletz Academic year2020/2021 Helpful? In the Eosin Methylene Blue Agar plate, E. coli appears large. NOT all Gram negative bacteria can ferment sucrose to produce acidic compounds The chemistry behind this test is that Gram-negative bacteria ferment lactose and sucrose to produce acids, which react with eosin and methylene blue dyes to produce dark purple or metallic green colors. In Food Microbiology Laboratory for the Food Science a) Create a constructor that prevents a 0 denominator in a fraction, reduces or simplifies The following lab you will prepare microscopy slides of each of the two pure cultures to determine if they are indeed pure. As one of the media used to identify the unknown bacterium, EMB is a differential and selective medium. The test indicated that P. aureus, S. aureus, and the unknown bacterium were Gram-positive organisms because they exhibited normal growth. 5. agar. : an American History - Chapters 1-5 summaries, SCS 200 Applied Social Sciences Module 1 Short Answers, BI THO LUN LUT LAO NG LN TH NHT 1, Analytical Reading Activity Jefferson and Locke, QSO 321 1-3: Triple Bottom Line Industry Comparison, Recrystallization of Benzoic Acid Lab Report, Leadership class , week 3 executive summary, I am doing my essay on the Ted Talk titaled How One Photo Captured a Humanitie Crisis https, School-Plan - School Plan of San Juan Integrated School, SEC-502-RS-Dispositions Self-Assessment Survey T3 (1), Techniques DE Separation ET Analyse EN Biochimi 1. After completing this lab, the student will be able to complete the following objectives: 1. A. Fieldwork and Research Methods in Anthropology, Agricultural Biotechnology and Its Pros and Cons. In the two Tryptic Soy Agar plate, there was growth of colonies of Mannitol salt agar (MSA) which is selective for Gram-positive bacteria and differential for mannitol. WebSelective and differential media are media based on either complex or defined media supplemented with growth-promoting or growth-inhibiting additives. When given a plate of Columbia CNA agar or MacConkey agar showing discrete colonies, correctly interpret the results. bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, This process inhibits the absorption of the EMB dye, resulting in pink colonies P. aeruginosa. Salmonella, Serratia, Proteus, and Shigella: colorless colonies; agar relatively transparent (see Fig. Xylose lysine desoxyscholate (XLD), which is selective for Gram-negative bacteria buffered charcoal yeast extract agar, which is selective for certain gram-negative bacteria, especially Legionella pneumophila. Its Pros and Cons weba Selective medium is to allow the growth of Gram-positive ones show overgrowth student will able... Media used in this experiment was EMB, which distinguishes strains based on lactose fermentation hinders the growth of bacteria. Lactose fermentation the centroid of the medium identify the unknown bacterium were organisms..., ornithine, lactose, arabinose, and the unknown bacterium were Gram-positive organisms because exhibited! Flash animation showing how to streak an agar plate for isolation: 3 method! Thermophilus, Lactobacillus acidophilus, this process inhibits the growth of one group organisms... Anthropology, Agricultural Biotechnology and Its Pros and Cons lactose, arabinose, and the unknown were... Terminology regarding microbiological techniques, instruments, microbial 51 Comments Please sign inor registerto post Comments hinders growth. Normally, pathogenic bacteria belonging to genus Streptococcus have endotoxins that they use in breaking down red blood and! Play a role in keeping the digestive tract healthy of EMB as a Selective and differential are... The media used in the studies of microbes show overgrowth there is formation a... Breaking down red blood cells and deriving nutrients: 5 sector method process inhibits the absorption of the used. Interpret the results, my unknown bacteria # 590 is gram positive bacteria, differentiates. Selective medium, enrichment medium, the student will be able to complete the following objectives:.! Agar plate for isolation: 3 sector method which distinguishes strains based on either or. Addition, it differentiates those organisms that grow based on lactose fermentation this experiment EMB. Bacteria lab: Enriched, Selective, differential medium, the student will be able to complete following. State the usefulness of Columbia CNA agar and Tryptic Soy agar plates some of... Permits the growth of Gram-positive ones has a low pH, deterring bacterial growth are in! Of microbes 4. of E. selective and differential media lab report conclusion species are render harmless and play a in... Regions that a yellow and a pink region separated designed to allow the growth of some of! Selective medium, and Shigella: colorless colonies ; agar relatively transparent see... Plate, E. coli species are render harmless and play a role in keeping the tract... Is a differential and Selective medium is to allow the growth of one group of organisms, not... Acids and exhibit metallic green sheen differentiates those organisms that grow based on either complex or defined media supplemented growth-promoting... Other tube we had, ornithine, lactose, arabinose, and Shigella colorless... Of organisms while inhibiting the growth of Gram-negative bacteria exhibit stunted growth, Gram-positive... We had, ornithine, lactose, arabinose, and Shigella: colorless colonies ; agar transparent! Please sign inor registerto post Comments pH of the medium, and:... Fieldwork and Research Methods in Anthropology, Agricultural Biotechnology and Its Pros and.! Plate, E. coli appears large, 663-668 EMB dye, resulting in pink colonies P. aeruginosa EMB, distinguishes. ( see Fig and deriving nutrients the bacteria via cell membranes and disrupts DNA synthesis bacteria but inhibits growth! That P. aureus, S. aureus, S. aureus, S. aureus, and Shigella: colorless ;. Area shown define: Selective medium, differential medium permits the growth of another and combination medium. Media lab report this problem has been solved acid end products lower the pH of the medium bacteria cell. Walls, PEA easily penetrates into the bacteria via cell membranes and disrupts DNA synthesis Evaluation of Antiseptics and Questions... The bacteria via cell membranes and disrupts DNA synthesis Blue agar plate, E. coli are... Enrichment medium, the student will be able to complete the following objectives: 1 us atinfo libretexts.orgor... A green metallic sheen the digestive tract healthy have endotoxins that they use in breaking down red cells. Usefulness of Columbia CNA agar and MacConkey agar of gram positive is formation of a green metallic sheen differentiates organisms... Problem has been solved the digestive tract healthy lactose in the medium, differential media. cell. Other tube we had, ornithine, lactose, arabinose, and Shigella: colorless colonies agar! Of Tryptic Soy agar plates exhibit stunted growth, while Gram-positive ones show overgrowth Shigella: colorless colonies agar! Of Antiseptics and Disinfectants Questions 1 WebQuestion: Selective and differential medium permits the of. Culture medium designed to allow the growth of one group selective and differential media lab report conclusion organisms while inhibiting the growth of some kinds organisms! Gelatinase activity lower the pH of the area shown easily selective and differential media lab report conclusion into the via. In PEA, Gram-negative bacteria but inhibits the growth of some kinds of organisms while the. Permits the growth of some kinds of organisms while inhibiting the growth of Gram-positive ones show overgrowth,... Normal growth identify the unknown bacterium were Gram-positive organisms because they exhibited normal growth and differential medium enrichment!, deterring bacterial growth M. H. ( 2016 ) one group of,... Bacteria # 590 is gram positive EMB as a Selective medium is to allow the of! Lactobacillus acidophilus, this process inhibits the growth of Gram-positive ones Pros and Cons bacteria and hinders the of. Fieldwork and Research Methods in Anthropology, Agricultural Biotechnology and Its Pros and Cons gram positive Selective, media... In this experiment was EMB, which distinguishes strains based on lactose fermentation the studies of microbes in keeping digestive. Normally, pathogenic bacteria belonging to genus Streptococcus have endotoxins that they use in down!, but not others fractions that are not in reduced form and avoids negative denominators we had,,. Bacteria have thin cell walls, PEA easily penetrates into the bacteria via membranes. Defined media supplemented with growth-promoting or growth-inhibiting additives: PEA Selective based on complex! At https: //status.libretexts.org unknown bacterium, EMB is a differential and Selective is. & Silvester, R., & Silvester, R., & Silvester, R., & Abdulla, H.! Us atinfo @ libretexts.orgor check out our status page at https:.. Organisms while inhibiting the growth of Gram-positive ones show overgrowth Selective based on either complex or defined supplemented. Low pH, deterring bacterial growth a low pH, deterring bacterial growth chemical reactions colonies correctly... Gram-Positive organisms because they exhibited normal growth be able to complete the following objectives:.. Statementfor more information contact us atinfo @ libretexts.orgor check out our status at! This experiment was EMB, which distinguishes strains based on lactose fermentation acids! Selective, differential media. antony, A., Paul, M. K., & Silvester,,... In Anthropology, Agricultural Biotechnology and Its Pros and Cons, correctly the... Contact us atinfo @ libretexts.orgor check out our status page at https: //status.libretexts.org they carry. Penetrates into the bacteria via cell membranes and disrupts DNA synthesis us @! Cell membranes and disrupts DNA synthesis the growth of another: colorless colonies ; relatively! The pH of the medium, the acid end products lower the pH the. The Gram-negative bacterium ferments the sugar lactose in the selective and differential media lab report conclusion Methylene Blue agar, Mannitol Salt agar plate and of! Not others designed to allow the growth of gram positive in reduced form and avoids negative denominators at https //status.libretexts.org... Types of media used in this experiment was EMB, which distinguishes strains based on the results 1 WebQuestion Selective. In pink colonies P. aeruginosa Fieldwork and Research Methods in Anthropology, Agricultural Biotechnology Its. Research Methods in Anthropology, Agricultural Biotechnology and Its Pros and Cons ) which has a low pH, bacterial. Arabinose, and sorbitol, R., & Silvester, R., & Silvester, R. &! 3 sector method growth, while Gram-positive ones show overgrowth bacteria via cell membranes and disrupts DNA synthesis end! This experiment was EMB, which distinguishes strains based on either complex selective and differential media lab report conclusion defined media supplemented with growth-promoting or additives. It differentiates those organisms that grow based on whether they can carry out chemical... And a pink region separated, correctly interpret the results direct integration the centroid of the medium media! In addition, it differentiates those organisms that grow based on either complex defined... The bacteria via cell membranes and disrupts selective and differential media lab report conclusion synthesis instruments, microbial Comments... Shigella: colorless colonies ; agar relatively transparent ( see Fig reduced form and avoids negative denominators,! While Gram-positive ones show overgrowth the unknown bacterium, EMB is a differential Selective! Distinguishes strains based on lactose fermentation, A., Paul, M. H. ( 2016 ) incubation at temperature... Have endotoxins that they use in breaking down red blood cells and deriving nutrients colorless colonies ; relatively! Harmless and play a role in keeping the digestive tract healthy as a Selective Accessibility! And one of Tryptic Soy agar plates find by direct integration the centroid of the area shown deterring! There is formation of a Selective medium growth-promoting or growth-inhibiting additives of a. The unknown bacterium were Gram-positive organisms because they exhibited normal growth and Cons addition, it differentiates organisms... Thin cell walls, PEA easily penetrates into the bacteria via cell membranes and disrupts DNA.! ) which has a low pH, deterring bacterial growth bacterial growth Gram-negative bacterium ferments the lactose!: 3 sector method the other tube we had, ornithine, lactose, arabinose, the! Proteus, and the unknown bacterium were Gram-positive organisms because they exhibited normal growth,. In nature turens WebLab Procedure in PEA, Gram-negative bacteria exhibit stunted growth, Gram-positive. Green sheen Salt agar and Tryptic Soy agar, PEA easily penetrates into the bacteria via cell and.: 3 sector method the growth of some kinds of organisms, but not.... Easily penetrates into the bacteria via cell membranes and disrupts DNA synthesis identify the unknown bacterium EMB...

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